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TaqMan™ Advanced miRNA Human A and B 96-well Plates, fast

详细信息

  • The Applied Biosystems TaqMan Advanced miRNA Human A and B 96-Well Plates, Fast, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format. This product contains a set of 8 plates for profiling of up to 752 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.

    Features of the TaqMan Advanced miRNA Human A and B 96-Well Plates include:
    • Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
    • Sample savings—requires only 4 μL of purified plasma/serum or 2–20 ng of total RNA per sample
    • Updated content—assay content updated for mirBase v.21
    • Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
    • Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

    Product details:
    Number of plates: 8
    Number of unique miRNAs (not including exogenous controls): 752
    Exogenous controls: cel-miR-39-3p, ath-miR159a
    Endogenous control: hsa-miR-16-5p
    Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
    Recommended master mix: TaqMan Fast Advanced Master Mix

    Notes
    • TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
    • These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.

Detection Method

  • Primer-Probe Detection

For Use With (Equipment)

  • 7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOnePlus™ System, ViiA™ 7 System

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