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Maxima SYBR Green/ROX qPCR Master Mix (2X)

详细信息

  • Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis. 
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    Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
    Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).
    Highlights
    • SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
    • Sensitivity—detects low copy number targets
    • Wide linear range—accurate quantification across 9 orders of magnitude
    • Reproducibility and convenience—ready-to-use 2x master mix
    Applications
    • Gene expression
    • siRNA validation
    • Genotyping
    • Pathogen detection
    Includes
    Maxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.
    Related Products
    Maxima SYBR Green/ROX qPCR Master Mix (2X)
    Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
    Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Detection Method

  • SYBR

For Use With (Equipment)

  • 7000 System, 7300 System, 7700 System, 7900HT System, StepOne™ System, StepOnePlus™ System

Reaction Speed


PCR Method


Sample Type (General)


Number of Reactions


Polymerase


Primer-Probe Compatibility


Product Line


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Sample Type (Specific)


Shipping Condition