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BCL11A cistrome in mouse erythroleukemia (MEL) cells
External Reference: GSE28334    
Measurement Type: Transcription Factor Binding (ChIP-Seq) Factors: Genetic Characteristics
Contact(s): Stuart Orkin
Curator(s): Emily Merrill
Submitted Date: 11/12/2014

summary

ChIP-seq analyses were performed in MEL cells expressing BirA alone or BirA and FLAG-Biotin tagged BCL11A (XL isoform). BCL11A chromatin occupancy in MEL cell line.


Growth Protocol

MEL cells were maintained in DMEM supplemented with 10% FCS at 37C incubator.

Treatment Protocol

Cells were fixed in 1% formaldehyde for 5 min at room temperature.

Extraction Protocol

Name

Input DNA


Organism

Mus musculus


Cell type

MEL (mouse erythroleukemia cell)


Disease state


Platform

Helicos HeliScope Single Molecule Sequencer



citations