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Vectors And Methods For Targeted Integration In Loci Comprising Constitutively Expressed Genes
公开时间: May 19, 2016
申请时间:
申请号:
Patent Application

WO_2016_074016_A1

摘要

The invention relates to a vector comprising: a 5' nucleic acid that is homologous to a genomic sequence 5' of a stop codon of a constitutively expressed gene; an exogenous nucleic acid; a 3' nucleic acid that is homologous to a genomic sequence 3' of the stop codon of the constitutively expressed gene; a translation interruption-reinitiation signal operably linked to the 5' nucleic acid and the exogenous nucleic acid, wherein the translation interruption-reinitiation signal is capable of replacing the stop codon of the constitutively expressed gene.


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细胞器官:

Schwann cell    cord blood   

方法技术:

cloning    DNA sequencing    image analysis    ionization time-of-flight mass spectrometry   

基因:

Ptch2 gene   

免疫治疗:

Morphogenesis    Monocyte