The Applied Biosystems TaqMan Advanced miRNA Human B 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains a set of 4 plates for profiling of up to 376 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.
Features of the TaqMan Advanced miRNA Human B 96-Well Plates include:
—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
• Sample savings
—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
• Updated content
—assay content updated for mirBase v.21
• Easy to use
—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
• Gold-standard TaqMan qPCR chemistry
—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families
Number of plates:
Number of unique miRNAs (not including exogenous controls):
Compatible with the following Applied Biosystems qPCR instruments:
Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit
, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
• For profiling of miRNAs using miRNA-specific RT, we recommend the TaqMan Array Human MicroRNA B Cards v3.0