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RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis (mouse data)

External Reference: GSE40171
Measurement Type: Transcription Profiling (RNA-Seq) Factors: Cell Type/Cell Line, Other (In Sample Notes), Culture Conditions

Contact(s): Sridhar Ramaswamy
Curator(s): Emily Merrill
Submitted Date: 01/07/2015

summary

Expression profiling of primary tumor, circulating tumor cells and ascites in a mouse model of pancreatic cancer suggested WNT signaling plays a role in pancreatic cancer metastasis. Induction of Wnt2 signaling in mouse pancreatic NB508 cells supported the hypothesis.

Growth Protocol

Treatment Protocol

Extraction Protocol

Qiagen RNeasy MinElute kit followed by Helicos single-molecule sequencing. See Pubmed IDs 18388294, 20133332.

Name

NB508-Vec_Sphere

Organism

Mus musculus

Cell type

NB508 (murine pancreatic cell line)

Disease state

Platform

Helicos HeliScope Single Molecule Sequencer

citations

RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis. Yu M, Ting DT, Stott SL, Wittner BS, Ozsolak F, Paul S, Ciciliano JC, Smas ME, Winokur D, Gilman AJ, et al. Nature. 2012 Jul 26; 487(7408):510-3. PMID: 22763454

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