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BCL11A cistrome in mouse erythroleukemia (MEL) cells

External Reference: GSE28334
Measurement Type: Transcription Factor Binding (ChIP-Seq) Factors: Genetic Characteristics

Contact(s): Stuart Orkin
Curator(s): Emily Merrill
Submitted Date: 11/12/2014

summary

ChIP-seq analyses were performed in MEL cells expressing BirA alone or BirA and FLAG-Biotin tagged BCL11A (XL isoform). BCL11A chromatin occupancy in MEL cell line.

Growth Protocol

MEL cells were maintained in DMEM supplemented with 10% FCS at 37C incubator.

Treatment Protocol

Cells were fixed in 1% formaldehyde for 5 min at room temperature.

Extraction Protocol

Name

Input DNA

Organism

Mus musculus

Cell type

MEL (mouse erythroleukemia cell)

Disease state

Platform

Helicos HeliScope Single Molecule Sequencer

citations

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