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Characterization of midgut stem cell- and enteroblast-specific Gal4 lines in drosophila.
Genesis, Issue: 10, Volume: 48, Pages: 607-611. | 2010-10-01
0 Patent citations    40 Scholarly citations     Reference Count: 20
Xiankun Zeng;Chhavi Chauhan;Steven X Hou

摘要

The homeostasis of Drosophila midgut is maintained by multipotent intestinal stem cells (ISCs), each of which gives rise to a new ISC and an immature daughter cell, enteroblast (EB), after one asymmetric cell division. In Drosophila, the Gal4-UAS system is widely used to manipulate gene expression in a tissue- or cell-specific manner, but in Drosophila midgut, there are no ISC- or EB-specific Gal4 lines available. Here we report the generation and characterization of Dl-Gal4 and Su(H)GBE-Gal4 lines, which are expressed specifically in the ISCs and EBs separately. Additionally, we demonstrate that Dl-Gal4 and Su(H)GBE-Gal4 are expressed in adult midgut progenitors (AMPs) and niche peripheral cells (PCs) separately in larval midgut. These two Gal4 lines will serve as invaluable tools for navigating ISC behaviors. genesis 48:607–611, 2010. Published 2010 Wiley-Liss, Inc.


机构

National Institutes of Health;Mouse Cancer Genetics Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland, USA. zengx2@mail.nih.govNational Institutes of HealthNational Institutes of Health


文献类型:

research support, n.i.h., intramural;journal article;

出版商:

Wiley-Liss Inc.

发表时间:

2010-10-01

基金信息

Intramural NIH HHS()United States