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Use of cell type-specific transcriptome to identify genes specifically involved in Müller glia differentiation during retinal development.
Developmental Neurobiology, Issue: 4, Volume: 74, Pages: 426-437. | 2014-01-04
0 Patent citations    2 Scholarly citations     Reference Count: 35
Yujin Mochizuki;Atsumi Iida;Eli Lyons;Ryoichiro Kageyama;Hiromitsu Nakauchi;Akira Murakami;Sumiko Watanabe

摘要

Retinal progenitor cells alter their properties over the course of development, and sequentially produce different sub-populations of retinal cells. We had previously found that early and late retinal progenitor cell populations can be distinguished by their surface antigens, SSEA-1 and c-kit, respectively. Using DNA microarray analysis, we examined the transcriptomes of SSEA-1 positive cells at E14, and c-kit positive, and c-kit negative cells at P1. By comparing data, we identified genes specifically expressed in c-kit positive late retinal progenitor cells. The previous literature suggests that most of the c-kit positive cell-specific genes are related to glia differentiation in brain or are expressed in Muller glia. Since Notch signaling promotes Muller glia differentiation in retina, we examined the effects of gain- and loss-of-Notch signaling on expression of these genes and found that all the genes were positively affected by Notch signaling. Finally, we screened the genes for their function in retinal development by shRNA-based suppression in retinal explants. In about half the genes, Muller glia differentiation was perturbed when their expression was suppressed. Taken together, these results show that at P1, c-kit positive retinal progenitor cells, which include Muller glia precursor cells, are enriched for genes related to glial differentiation. We propose analysis of purified subsets of retinal cells as a powerful tool to elucidate the molecular basis of retinal development. © 2013 Wiley Periodicals, Inc. Develop Neurobiol 74: 426–437, 2014


机构

University of Tokyo;Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan; Department of Ophthalmology, Graduate School of Medicine, Juntendo University, Tokyo, Japan.;Department of Molecular and Developmental Biology; Institute of Medical Science, University of Tokyo; Tokyo Japan;Department of Ophthalmology; Graduate School of Medicine; Juntendo University; Tokyo JapanUniversity of Tokyo;Department of Molecular and Developmental Biology; Institute of Medical Science, University of Tokyo; Tokyo JapanUniversity of Tokyo;Department of Molecular and Developmental Biology; Institute of Medical Science, University of Tokyo; Tokyo JapanKyoto University;Institute for Virus Research; Kyoto University; Kyoto JapanUniversity of Tokyo;Division of Stem Cell Therapy; Institute of Medical Science; University of Tokyo; Tokyo JapanJuntendo University;Department of Ophthalmology; Graduate School of Medicine; Juntendo University; Tokyo JapanUniversity of Tokyo;Department of Molecular and Developmental Biology; Institute of Medical Science, University of Tokyo; Tokyo Japan


文献类型:

journal article;research support, non-u.s. gov't;

出版商:

John Wiley and Sons Inc.

发表时间:

2014-01-04

基金信息