干细胞领域知识发现平台

提升科研信息化水平
支撑研究所科技创新

  融合多源信息    打通数据孤岛
  挖掘知识关联    放大数据价值
  集成知识计算    促进知识发现

免费注册账号
查看全文和研发动态、知识导航以及收藏您喜欢的数据等操作,您需要注册一个免费帐户并登录。

写笔记

您最近的笔记
Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a novel stem cell gene.
Developmental Biology, Issue: 1, Volume: 293, Pages: 90-103. | 2006-01-05
0 Patent citations    29 Scholarly citations     Reference Count: 63
Claire E Hirst;Elizabeth S Ng;Lisa Azzola;Anne K Voss;Tim Thomas;Edouard G Stanley;Andrew G Elefanty

摘要

We analyzed the transcriptional profiles of differentiating mouse embryonic stem cells (mESCs) and show that embryoid bodies (EBs) sequentially expressed genes associated with the epiblast, primitive streak, mesoderm and endoderm of the developing embryo, validating ESCs as a model system for identifying cohorts of genes marking specific stages of embryogenesis. By comparing the transcriptional profiles of undifferentiated ESCs to those of their differentiated progeny, we identified 503 mESC and 983 hESC genes selectively expressed in undifferentiated ES cells. Over 75% of the mESC genes were expressed in hESC and vice versa, attesting to the underlying similarity of mESCs and hESCs. The expression of a cohort of 68 genes decreased greater than 2-fold during differentiation in both mESCs and hESCs. As well as containing many validated ESC genes such as Oct4 [Pou5f1], Nanog and Nodal, this cohort included an uncharacterised gene (FLJ30046), which we designated SLAIN1/Slain1. Slain1 was expressed at the stem cell and epiblast stages of ESC differentiation and in the epiblast, nervous system, tailbud and somites of the developing mouse embryo. SLAIN1 and its more widely expressed homologue SLAIN2 comprise a new family of structurally unique genes conserved throughout vertebrate evolution.


机构

Monash University, Clayton campus;Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, VIC 3800, Australia.Monash University, Clayton campusMonash University, Clayton campusWalter and Eliza Hall Institute of Medical ResearchWalter and Eliza Hall Institute of Medical ResearchMonash University, Clayton campusMonash University, Clayton campus


文献类型:

journal article;comparative study;research support, non-u.s. gov't;

出版商:

Academic Press Inc.

发表时间:

2006-01-05

基金信息