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Evidence of a Sophisticatedly Heterogeneous Population of Human Umbilical Vein Endothelial Cells
Transplantation Proceedings, Issue: 4, Volume: 46, Pages: 1251-1253. | 2014-01-05
0 Patent citations    1 Scholarly citations     Reference Count: 5
Yun-Wen Zheng;Yun-Zhong Nie;Tomonori Tsuchida;Ran-Ran Zhang;K Aoki;Keisuke Sekine;M Ogawa;Takanori Takebe;Yasuharu Ueno;H Sakakibara;F Hirahara;Hideki Taniguchi

摘要

Induction and promotion of angiogenesis play a role in a diverse range of physiologic and pathophysiologic processes that are especially relevant to the field of regenerative medicine. For assessing vasculogenesis and neo-angiogenesis, identifying angiogenic factors, angiocrine factors, and vascular niche, facilitating tissue-repair and tumor growth, efficiently generating induced pluripotent stem cells, and coculturing with organ-specific stem cells, isolation and characterization of the subpopulation of human umbilical vein endothelial cells (HUVECs) and their endothelial progenitor cells (EPCs) are needed. In this study, primary HUVECs were collected from fresh umbilical cords and fractionated and characterized with the use of flow cytometry. Clonal colony assay showed that endothelial colony-forming units in culture frequently existed in fresh HUVECs. Antigenic profiling demonstrated that undifferentiated EPCs in HUVECs had normal endothelial marker CD31 with a subpopulation of cells positive for hematopoietic stem cell marker CD34 and c-Kit. With continuing passages, EPC markers CD34 and vascular endothelial growth factor receptor 2 expression decreased dramatically. Moreover, a distinct subpopulation with different proliferative capability and angiogenesis from the early-passage HUVECs was shown. In conclusion, it is possible to isolate accurately and to enrich EPCs or hematoangioblast-like cells from a heterogeneous population of HUVECs, and to explore the differential process with flow cytometry for further investigations.


机构

Yokohama City University;Department of Regenerative Medicine, Yokohama City University School of Medicine, Yokohama, Japan.Yokohama City University;Department of Regenerative Medicine, Yokohama City University School of Medicine, Yokohama, Japan.Yokohama City University;Department of Regenerative Medicine, Yokohama City University School of Medicine, Yokohama, Japan.Yokohama City University;Department of Regenerative Medicine, Yokohama City University School of Medicine, Yokohama, Japan.Yokohama City University;Medical Course, Yokohama City University School of Medicine, Yokohama, Japan.Yokohama City University;Department of Regenerative Medicine, Yokohama City University School of Medicine, Yokohama, Japan.Yokohama City University;Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, Yokohama, Japan.Yokohama City University;Department of Regenerative Medicine, Yokohama City University School of Medicine, Yokohama, Japan.Yokohama City University;Department of Regenerative Medicine, Yokohama City University School of Medicine, Yokohama, Japan.Yokohama City University;Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, Yokohama, Japan.Yokohama City University;Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, Yokohama, Japan.Yokohama City University;Department of Regenerative Medicine, Yokohama City University School of Medicine, Yokohama, Japan. Electronic address: rtanigu@med.yokohama-cu.ac.jp.


文献类型:

journal article;research support, non-u.s. gov't;

出版商:

Elsevier USA

发表时间:

2014-01-05

基金信息