The present invention provides a method for improving traits in a plant, like e.g. improving yield-related traits like number of flowers, number of siliques, seed yield, stem growth in a plant, the method comprising disruption of endogenous ROCK1 gene in a plant cell, wherein said disruption inhibits expression and/or activity of a product of said endogenous ROCK1 gene compared to a corresponding control plant cell lacking such a disruption.
Methods are disclosed for non-invasively obtaining fetal cells from a pregnant female. The methods include placing an absorbent medium in an interlabial or intravaginal space or adjacent to the perineum at the vaginal opening of the pregnant female, and collecting vaginal fluid comprising cells in the absorbent medium while the absorbent medium is interlabial or intravaginal space or adjacent to the perineum at the vaginal opening. The absorbent medium is removed and cells are isolated from the absorbent medium to obtain the fetal cells. The fetal cells can be, for example, somatic cells, embroyic stem cells, fetal stem cells or trophoblast cells.
The purpose of the present invention is to provide immortalized stem cells, which produce a growth factor capable of regenerating various kinds of tissues that have been damaged by a variety of causes, and a method for producing the aforesaid immortalized stem cells. Another purpose of the present invention is to provide a medicinal composition and a medicinal preparation for restoring damaged tissues, and a method for the percutaneous absorption of a culture supernatant. Provided are immortalized stem cells that are obtained by isolating stem cells selected from the group consisting of mammalian mesenchymal cells, an embryo at the early stage of the development and somatic cells, first culturing the cells to give first stage culture cells, transferring four kinds of genes into the first stage culture cells to give transgenic cells, and selecting the desired immortalized stem cells from among the transgenic cells using the expression of STRO-1 as an index. Also provided are a medicinal composition and a medicinal preparation for restoring damaged tissues which comprise a culture supernatant of the immortalized stem cells as the active ingredient.
Disclosed is a pacemaker cell derived from an embryonic stem cell (ES cell) or an induced pluripotent stem cell (iPS cell), that is provided with an HCN4 channel and an Na channel and is capable of controlling heart rates by controlling the Na channel. Also disclosed is a cardiac pacemaker that comprises the abovementioned pacemaker cell.
The subject invention concerns new methods which make it possible, for the first time, to grow functional islet-producing stem cells (IPSCs), islet progenitor cells (IPCs) and IPC-derived islets (IdIs) in in vitro cultures. The subject invention also concerns the use of the in vitro grown IPSCs, IPCs and/or IdIs for implantation into a mammal for in vivo therapy of diabetes. The subject invention further concerns a process of using the implanted cells for growing a pancreas-like structure in vivo that has the same functional, morphological and histological characteristics as those observed in normal pancreatic endocrine tissue. The ability to grow these cells in vitro and pancreas-like structures in vivo opens up important new avenues for research and therapy relating to diabetes.
The invention relates to methods for treating hair graying. More specifically, the present invention discloses new methods for reducing, stopping and/or reversing of hair graying in mammals by stimulating stem cell migration to produce or stimulate melanocytes in hair follicles. The invention further teaches a stem cell mobilizing composition for treating graying hair in a subject, comprising blue-green algae, which stimulates stem cell mobilization and growth of melanocytes in hair follicles.
The present invention relates to methods for identifying, assessing, preventing, and treating cancer (e.g., lymphoid and/or myeloid malignancies such as B-ALL in humans). A variety of histone H3K27rne3 biomarkets are provided, wherein alterations in the copy number of one or more of the bioniarkers and/or alterations in the amount, structure, and/or activity of one or more of the biomarkers is associated with cancer status and indicates amenability to treatment or prevention by modulating H3K27me3 levels. The present invention further relates to methods of increasing the number of lymphoid progenitor cells (e.g., increase self-renewal and cell proliferation) by contacting the lymphoid progenitor ceils (e.g., wild type and/or genomicaily altered cells) with an agent that inhibits polycomb repressor complex 2 (PRC2) activity or reduces H3K27roe3 levels.
The present invention relates to applications based on the findings of the interaction between NKp80 and its ligand AICL. The use of substances blocking the interaction between NKp80 and AICL is disclosed, for the manufacture of a medicament for the prevention or treatment of inflammatory diseases, in particular autoimmune diseases. Preferably, the substance is selected from the group comprising anti-NKp80- antibodies, anti-AICL-antibodies, soluble NKp80, soluble AICL, or functional fragments thereof.
The present invention provides methods of modulating neurite outgrowth in an animal. The methods comprise a general administration of galanin-3 receptor antagonists under conditions sufficient to produce neurite outgrowth.
The present invention provides human neural crest stem cells which are pluripotent, self-renewing, and can be maintained as a stable cell line via retrovirus-mediated vmyc gene transfer. These human neural crest stem cells and their daughter progeny cells are uncommitted and undifferentiated cells which can subsequently be induced to a range of alternative recognized types of differentiated and functional cells including neurons, Schwann cells, adrenal chromaffin cells, and skeletal muscle cells. These human neural crest stem cells and their progeny cells are suitable for implantation in-vivo for cell therapy treatment of human neurological disorders and diseases.