Methods are disclosed for forming bone and/or cartilage in an avian subject. The methods include administering to the avian subject a therapeutically effective amount of a composition comprising avian mesenchymal stem cells and a hydrogel that supports the differentiation of the avian mesenchymal stem cells into cells of an osteogenic and/or condrogenic lineage. In some embodiments, methods are disclosed for repairing a bone defect and preventing infection, such as that associated bone fracture, in an avian subject. The methods include administering locally to the bone defect a composition comprising a therapeutically effective amount of avian mesenchymal stem cells and a hydrogel, such as a methacrylated gelatin hydrogel.
Dextran sulfate in a range of 3500 and 9500 Da is employed to mobilize cells, such as stem and/or progenitor cells and certain white blood cells, in particular lymphocytes, into the peripheral blood of a subject. Dextran sulfate has a very fast cell mobilizing effect that implies that any cell harvest can be started almost immediately following dextran sulfate administration.
The present disclosure provides methods for generating enhanced affinity T cell receptors by agonist selection of hematopoietic progenitor cells expressing an antigen specific TCRalpha cultured with stromal cells expressing Delta-like-1 or Delta-like-4, compositions prepared from such methods, and uses of thereof.
The present invention relates to a novel transgenic plant having tolerance to salt stress. The plant is transformed with a recombinant nucleic acid encoding glutamic acid decarboxylase isolated from Or?za sativa. Still further it also relates to a method of producing the transgenic plants that are salt tolerant.
We describe a cell culture substrate comprising a polymerised high internal phase emulsion polymer adapted and modified for use in the routine culture of cells in three dimensions; typically mammalian cells and the use of the substrate in a cell culture system for investigation and analysis of proliferation, differentiation and function of cells.
The present invention provides cells, tissues or organs for use in cell therapy or xenotransplantation in which at least one gene comprising an antigenic determinant recognized by a recipient organism has been disrupted. The present invention also includes methods of administering such cells and transplanting such tissues or organs in which genes encoding antigenic determinants recognized by the recipient organism have been disrupted.
The invention provides isolated maize RAD51 nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering maize RAD51 levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.
The invention relates to in vitro methods for isolating and characterizing human salivary gland cells for stem cell transplantation, and to the therapeutic use of the cells, in particular for treating or preventing xerostomia.
The present invention refers to an animal model system which mimics the immunosuppressive environment found in cancer, autoimmune and infectious diseases, as well as in chronic inflammation. In said animal model system, which is an object of the present invention, sustained exposure to antigen induces TCR xi chain downregulation and impaired T cell function. Further objects of the present invention are the method of generating the animal model system, as well as methods of screening for substances that inhibit said xi chain downregulation. Finally, the present invention provides xi chain as a marker for the immunological status of a subject in need of immunotherapy.