Provided herein are methods for the efficient in vitro differentiation of HLA homozygous blood cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into HLA homozygous immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.
The present disclosure provides compositions and methods for promoting stem cell and/or progenitor cell proliferation and/or differentiation. The provided compositions may be useful in treating a disease or condition that is related to mucosal barrier function, e.g., wound healing, treating skin conditions (e.g., atopic dermatitis, psoriasis, bed sores, or condition related to the aging of skin), treating a lung disorders (e.g., asthma), a condition related to improving mucosal barrier function, and/or treating injury to GI mucosa in a subject in need thereof. The present disclosure also provides methods for promoting the proliferation and/or differentiation of stem cells and/or the progenitor cells in a subject in need of such treatment by administering a composition. The ability to stimulate the proliferation and/or differentiation of stem cells and/or the progenitor cells in vivo, ex vivo and/or in vitro provides tremendous benefit. The present disclosure can be used to increase stem cell populations in in vivo, ex vivo and/or in vitro. Stem cell transplantation provides treatments and/or cures of many disease states, degeneration and/or injury.
A cell preparation for preventing and/or treating a vascular disorder such as aortic aneurysm, said cell preparation comprising SSEA-3-positive pluripotent stem cells (Muse cells) derived from a mesenchymal tissue of a living body or cultured mesenchymal cells.
Provided is a method for detecting an intracellular endogenous protein using living cells. A method for identifying an intracellular endogenous protein, said method comprising the steps of: (1) a step for introducing, into a desired cell, intracellular endogenous protein-responsive mRNA that contains an aptamer sequence unique to an intracellular endogenous protein and also contains a marker gene sequence functionally bonded thereto, or a vector that codes said mRNA; and (2) a step for identifying the intracellular endogenous protein on the basis of the amount of translation of the marker gene.
A method using small molecule groups and multiple target points for targetedly inducing direct differentiation of human stem cells, such as human embryonic stem cells or induced pluripotent stem cells, toward hepatic cells. A culture medium for inducing targeted direct differentiation of human stem cells toward hepatic cells and a culturing method. The method requires neither the introduction of foreign genes into the stem cells, nor separate induction steps, nor various cell growth factors. The use of chemical small molecules is sufficient to achieve targeted direct differentiation of human stem cells toward hepatic cells. The obtained differentiated human hepatic cells have the typical characteristics of human hepatic cells; differentiated hepatic precursor cells may be passed on over long periods; and, differentiated mature hepatic cells may be passed on for a relatively limited period. The method involves conventional culturing, is easy to implement, low in cost, and safe and stable.
The purpose of the present invention is to provide an agent for improving skin looseness or aging caused by dermal cavitation. By attracting fat stem cells into cavities in the dermis, dermal fibroblasts around the cavities can be activated and thus dermal cavitation can be ameliorated. As the results of a screening for fat stem cell attractants, it was found that rosemary extract and licorice extract are capable of attracting fat stem cells and, therefore, can improve skin looseness or aging caused by dermal cavitation.
Provided are a mesenchymal stem cell, a clonogenic amplification method thereof, a separation method thereof and uses thereof. The mesenchymal stem cell expresses an insulin-like growth factor-1 receptor, and has a self-renewal capability and the capability for pluripotent differentiation.
Methods are disclosed for forming bone and/or cartilage in an avian subject. The methods include administering to the avian subject a therapeutically effective amount of a composition comprising avian mesenchymal stem cells and a hydrogel that supports the differentiation of the avian mesenchymal stem cells into cells of an osteogenic and/or condrogenic lineage. In some embodiments, methods are disclosed for repairing a bone defect and preventing infection, such as that associated bone fracture, in an avian subject. The methods include administering locally to the bone defect a composition comprising a therapeutically effective amount of avian mesenchymal stem cells and a hydrogel, such as a methacrylated gelatin hydrogel.
The invention relates to a vector comprising: a 5' nucleic acid that is homologous to a genomic sequence 5' of a stop codon of a constitutively expressed gene; an exogenous nucleic acid; a 3' nucleic acid that is homologous to a genomic sequence 3' of the stop codon of the constitutively expressed gene; a translation interruption-reinitiation signal operably linked to the 5' nucleic acid and the exogenous nucleic acid, wherein the translation interruption-reinitiation signal is capable of replacing the stop codon of the constitutively expressed gene.
The present invention provides a method for improving the efficiency of inducing a pluripotent stem cell, and a vector and composition used for the method. The inventors succeeded in significantly increasing the efficiency of inducing a pluripotent stem cell by further introducing a vector including a KLF gene and not including an OCT gene and a SOX gene in pluripotent stem cell induction including a step for introducing a vector including a KLF gene, an OCT gene, and a SOX gene in this order. This method is capable of efficiently inducing a pluripotent stem cell under temperature conditions closer to a physiological environment, and has excellent characteristics in that the vector is rapidly eliminated after pluripotent stem cell induction. The present invention makes it possible to induce a pluripotent stem cell with greater efficiency.