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Abstract

Methods are disclosed for forming bone and/or cartilage in an avian subject. The methods include administering to the avian subject a therapeutically effective amount of a composition comprising avian mesenchymal stem cells and a hydrogel that supports the differentiation of the avian mesenchymal stem cells into cells of an osteogenic and/or condrogenic lineage. In some embodiments, methods are disclosed for repairing a bone defect and preventing infection, such as that associated bone fracture, in an avian subject. The methods include administering locally to the bone defect a composition comprising a therapeutically effective amount of avian mesenchymal stem cells and a hydrogel, such as a methacrylated gelatin hydrogel.


Abstract

Embodiments herein provide methods, apparatuses, and systems for detecting, monitoring, measuring, and/or characterizing the activity of phosphoproteins, such as tyrosine kinases (TKs) and downstream proteins in TK signal transduction pathways (e.g., TK pathway proteins). In various embodiments, the methods, apparatuses, and systems may use nanoparticles, such as quantum dots (QD), to detect and/or characterize the abnormally overactive TK signaling pathways that underlie tumorgenesis and tumor progression. In various embodiments, the QD-based methods, apparatuses, and systems may have a sufficiently high degree of sensitivity to enable the identification of new TK signaling pathway markers, for example for use in diagnosing, staging, monitoring, and/or prognosing cancers, or in evaluating the efficacy of cancer therapeutics.


Abstract

The present invention is directed to the hTERT gene-transduced HUMAN Neural Stem Cells, the method for establishing and identifying them. The method comprise the following steps: (1) extracting human neural stem cells, incubating expansion in vitro so as to obtain neuron spheres i.e. neural stem cells; (2) packing the recombinant virus carrying the hTERT gene; (3) transfecting neural stem cells with the recombinant virus carrying the hTERT gene; and (4) passaging and expanding the hTERT gene-transduced human neural stem cells so as to obtain human neural stem cell lines. The telomerase activity of neural stem cells and the length of telomeres, the properties of neural stem cells were identified. The establish of neural stem cell lines was determined. The neural stem cells can be used as basic studies and the screen of neural system drugs, and have the important clinical values, can be used as the cell vector of gene therapy for neural system diseases, for the degenerative diseases of neural systems, as the cellular materials of transplantation for lesion repairing.

公开时间: 2012-04-19
发明人:FARAHAT WALEED AHMED US, ASADA HARUHIKO HARRY US, CHUNG SEOK, MACKLIS JEFFREY, LEE KWANG HO, KOTHAPALLI CHANDRASEKHAR, KAMM ROGER DALE US, KIM CHOONG, TU NGUYEN LE THANH, VARNER JOHANNA, THARIN SUZANNE, PARK YOUNG KUM SG, WOOD LEVI B US, ZERVANTONAKIS LOANNIS K US
知识棱镜:

Abstract

Provided herein are microfluidic devices that can be used as a 3D bioassay, e.g., for drug screening, personalized medicine, tissue engineering, wound healing, and other applications. The device has a series of channels {e.g., small fluid channels) in a small polymer block wherein one or more of the channels can be filled with a biologically relevant gel, such as collagen, which is held in place by posts. As shown herein, when the device is plated with cells such as endothelial cells, new blood vessels grow in the gel, which is thick enough for the cells to grow in three dimensions. Other channels, e.g., fluid channels, allow drugs or biological material to be exposed to the 3D cell growth. Cells, such as endothelial cells, can be cultured and observed as they grow on the surface of a 3D gel scaffold, where e.g., rates of angiogenesis can be measured, as well as intervascularization and extravascularization of cancerous cells.

公开时间: 2011-07-07
发明人:TVOROGOV DENIS FI, ALITALO KARI FI, ANISIMOV ANDREY FI, LEPPAENEN VELI-MATTI FI
知识棱镜:

Abstract

Provided herein are compositions comprising a first binding construct which specifically binds to a first epitope of a Receptor Tyrosine Kinase (RTK) and a second binding construct which binds to a second epitope of the RTK, wherein the second epitope is different from the first epitope, wherein each of the first and second binding constructs reduces ligand-induced activation of the RTK. In specific embodiments, the RTK is a receptor for a Vascular Endothelial Growth Factor (VEGF) or a Platelet-Derived Growth Factor (PDGF). Further provided herein are methods of inhibiting cellular activities, including inhibiting cell growth (e.g., tumor or cancer cell growth), which activities are mediated by the RTK. The methods comprise contacting an RTK-expressing cell with the composition of the present disclosures.

公开时间: 2014-05-15
发明人:Yung Kin-lam, Li Hung-wing, Lui Nga-ping, Tsui Yat-ping, Ho See-lok, Chan Ying-shing, Shum Kwok-yan, Tsang Edman Shik-chi
知识棱镜:

Abstract

A method of extracting neural stem cells from a living subject, comprising the steps of introducing magnetic nanoparticles into the subject, targeting the neural stem cells with the magnetic nanoparticles to form magnetic nanoparticle-targeted cells, isolating the magnetic nanoparticle-targeted cells, extracting the magnetic nanoparticles-targeted cells from the subject.

公开时间: 2007-01-25
发明人:Sayre Chauncey B, Silva Francisco J, Pau Kwok-yuen F
知识棱镜:

Abstract

Pluripotent therapeutically programmed cells and methods for making such cells are provided. The pluripotent therapeutically programmed cells are post-natal stem cells which have been matured such that they represent either a more differentiated state or a less differentiated state after contact with stimulatory factors. The pluripotent therapeutically reprogrammed cells are suitable for cellular regenerative therapy and have the potential to differentiate into more committed cell lineages. Also disclosed are culture media for therapeutically reprogramming post-natal stem cells.

公开时间: 2014-09-04
发明人:DUDAKOV JARROD US, BRINK MARCEL VAN DEN US, HANASH ALAN US
知识棱镜:

Abstract

The present invention provides methods and compositions for the use of IL-22 to promote thymic growth following thymic insult. In particularly preferred embodiments, the present invention provides methods of using therapeutic IL-22 compositions for treating patients with thymic atrophy and alterations in bone marrow derived white blood cells, including cancer patients undergoing chemotherapy, patients exposed to radiation (i.e. cancer therapy, nuclear disaster, terrorist attack, etc.), patients with HIV infections/AIDS, patients with organ transplantation, aging patients, and the like. In a further embodiment, therapeutic IL-22 compositions are contemplated as a prophylactic to boost immune response when additional T-cell function is needed, i.e. to boost immune response during vaccination.


Abstract

This invention provides, a recombinant polypeptide encoding a chimera. The chimera includes a DNase I fragment or a homologue thereof and a Cdt fragment or a homologue thereof. Further, the invention provides methods, utilizing the recombinant polypeptide encoding the chimera, such as a method for inhibiting the proliferation of a neoplastic cell, a method for treating a neoplastic disease in a human subject, a method for inhibiting or suppressing a neoplastic disease in a human subject, and a method for reducing the symptoms associated with a neoplastic disease in a human subject.

公开时间: 2010-02-18
发明人:GEFFNER LUIS FIDEL EC, SILVA FRANCISCO US
知识棱镜:

Abstract

The disclosure provides compositions and methods useful for treating injuries to the central nervous system (e.g., spinal cord injuries). The compositions and methods described herein can be optionally used in combination with a variety of techniques (e.g., surgical techniques) and/or therapies (e.g., physical therapy regimens) to affect treatment of injuries to the central nervous system.

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