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公开时间: 2016-05-19
发明人:Schmülling Thomas, Niemann Michael, Tomàs Werner
知识棱镜:

Abstract

The present invention provides a method for improving traits in a plant, like e.g. improving yield-related traits like number of flowers, number of siliques, seed yield, stem growth in a plant, the method comprising disruption of endogenous ROCK1 gene in a plant cell, wherein said disruption inhibits expression and/or activity of a product of said endogenous ROCK1 gene compared to a corresponding control plant cell lacking such a disruption.

公开时间: 2014-08-07
发明人:KITAZAWA MANABU JP, KURIYAMA YOKO JP, SUGIMOTO NAO JP, SENDA SHO JP, OKAMOTO SATORU JP, YOSHIDA TOMOMI JP, HARADA IKUE JP
知识棱镜:

Abstract

Provided are a culture method for the undifferentiated proliferation of pluripotent stem cells, said method comprising a step for culturing the pluripotent stem cells in a culture medium that is characterized by containing at least one member selected from the group consisting of ethanolamine, ethanolamine analogs and pharmaceutically acceptable salts thereof and the concentration of [beta]-mercaptoethanol in the culture medium being substantially 0 or not greater than 9 [mu]M, etc.

公开时间: 2009-01-08
发明人:Claesson Hans Peter Ingemar, Tarrant Laurence J B, Smith Robert Lane, Shortkroff Sonya
知识棱镜:

Abstract

A double-structured tissue implant and a method for preparation and use thereof for implantation into tissue defects. The double-structured tissue implant comprising a primary scaffold and a secondary scaffold consisting of a soluble collagen solution in combination with a non-ionic surfactant generated and positioned within the primary scaffold. A stand alone secondary scaffold implant or unit. A process for preparation of the double-structured implant or the stand alone secondary scaffold comprising lyophilization and dehydrothermal treatment.


Abstract

The invention provides methods of detecting bacteria in fluids, including blood, platelets and other blood products for transfusion, and urine. The methods are based on lysing the bacteria to release ATP and detecting the ATP. Eukaryotic cell contamination is a problem to be overcome, because eukaryotic cell contain large amounts of ATP. Thus, some of the methods involve separating intact eukaryotic cells (e.g., platelets) from intact bacterial cells before lysing the bacterial cells to release ATP, contacting the ATP with an ATP-consuming enzyme that catalyzes a reaction, and monitoring the enzyme-catalyzed reaction. Typically, the enzyme is luciferin, and the reaction is monitored by detecting light produced by the luciferin. Other methods of the invention involve contacting a fluid sample with a support surface that binds bacterial cells, lysing the bacterial cells to release ATP, contacting the ATP with an ATP-consuming enzyme, and monitoring the enzyme-catalyzed reaction. Apparatuses for carrying out the methods are also disclosed.

公开时间: 2012-11-27
发明人:HARMON ALEXANDER M., HARRIS IAN ROSS, KIHM ANTHONY J., MESSINA DARIN J., MISTRY SANJAY, SEYDA AGNIESZKA, GOSIEWSKA ANNA
知识棱镜:

Abstract

Cells derived from human umbilical cords are disclosed along with methods for their therapeutic use. Isolation techniques, culture methods and detailed characterization of the cells with respect to their cell surface markers, gene expression, and their secretion of trophic factors are described.


Abstract

Synthetic Stem Cell-like Tissue Healing and Regeneration Medication with Anti-inflammatory, Protein Synthesis, Enzyme Deficiency Activation and Genetic Therapy, and Anti-cancer Agent derived from a series of inventions that include these products of Biomolecular Engineering, Drug Discovery from a Biologic Periodic Table of Applied Biochemistry and Biophysics. Tissue has a self healing effect promoting tissue healing and tissue regeneration. Not only does it maintain good health but also it has been observed that the patient's blood is withdrawn from the patient and applied to the ulcer has healing qualities. Cartilage placed in a wound promotes and accelerates wound healing. The anabolic biochemical and biophysical equivalent of tissue has been found in these embodiments to have the same pharmacologic qualities, when devoid of genetic DNA mismatch and other catabolic factors including the catabolic effects of microorganism overgrowth that lacks pro-biotic qualities. The healing efficacy of these tissue components gives us further appreciation of the protective action of human tissue over and above and other than the immune protective system or perhaps an integral component part of the immune system.


Abstract

A method to determine an engrafting cell dose of hematopoietic stem cell transplant units from transplant sources having nucleated cells selected from the group consisting of cord blood, bone marrow, peripheral blood comprising the steps ofsubjecting the source to a substantially complete erythrocyte lysis,measuring in a cell counter a signal corresponding selectively to white blood cells,assessing essentially quantitatively nucleated red blood cell (NRBC) count as part of the total nucleated cell (NC) count,and determining the number of white blood cells (WBCs) as transplant relevant cells.


Abstract

本发明涉及一种无机纳米材料层状双氢氧化物在小鼠胚胎干细胞培养中的应用。利用纳米层状双氢氧化物能够在不添加LIF因子的情况下,促进各多能性基因的表达,抑制细胞分化,并且处理后的细胞仍然具有向三个胚层分化的潜能。纳米层状双氢氧化物具有良好的生物相容性,低细胞毒性,易于接触细胞表面等特点,成本低廉,材料合成便捷,作用效果佳,为胚胎干细胞研究带来广泛的应用前景。

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